BioSpec 59014N 316 Stainless Steel BioPulverizer with Hammer, 1-10g Capacity

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Price: $347.00    

Product Feature

• Type 316 stainless steel hand-operated BioPulverizer
• Quickly pulverizing 1-10g of frozen tissue
• Hammer provided
• Requires access to dry iceor about 200 mL of liquid nitrogen
• Consists of a hole machined in a stainless steel base into which fits a special piston or pestle

Product Description

The BioPulverizer reduces tissue hard-frozen in liquid nitrogen to a fine powder. Because of the high water content, most fresh tissue becomes as brittle as glass at liquid nitrogen temperatures. A sharp blow with a hammer will shatter it into tiny pieces. Called freeze-fracturing or cryopulverization, the method is especially useful for preparing tough, fibrous tissues such as skin, cartilage, cornea, etc. for subsequent rapid cell disruption using other techniques. The Bio-Pulverizer consists of a hole machined in a stainless steel base into which fits a special piston, or pestle. In a typical procedure, up to 10 g of animal or plant tissue is hard-frozen in liquid nitrogen and placed in the pre-chilled BioPulverizer. The piston delivers a blow to the brittle tissue reducing it to powder. Freeze fracturing with the Bio-Pulverizer is also useful for extracting labile tissue metabolites. Place the BioPulverizer in a shallow container and cool thoroughly with either liquid nitrogen (preferred) or a dry-ice/alcohol mixture (next best). Place prefrozen tissue in the well of the mortar. Remove the mortar from the shallow container, place it on the lab bench and pulverize the tissue with one or two blows to the pestle with the hammer. The mass of the stainless steel will preserve the cold long enough for you to complete your pulverization without adding additional liquid nitrogen. Empty the powdered contents for subsequent homogenization or extraction procedures.

BioSpec 59014N 316 Stainless Steel BioPulverizer with Hammer, 1-10g Capacity Review

I use this to get rid of dead bodies in my apartment. It takes quite a while to cut them into pieces that will fit inside the cup, but after freezing the tissue with liquid nitrogen the hammer and pestle easily reduce most body parts to a mush that can be easily washed down the drain. Stainless steel makes cleanup a breeze.

I generally have to use a rotary grinder to powder the bones as they do not contain enough water for the liquid nitrogen to work properly on them. The liquid nitrogen only adds another couple hundred dollars per body. I made a deal with a guy who owns a delivery truck to fill a couple of 5 gallon buckets for me.

I gave this product four stars because its small size makes the job take a longer, usually 4 to 5 hours per body, and there's no built in mechanism to tell you when pulverisation is complete or easily cycle the contents. Sometimes the hammering melts the top layer a bit, so add a little more liquid nitrogen if it starts to splash up at you.

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